Competitive ELISA

The fourth type of ELISA is competitive ELISA. As the name suggest there is going to be a competition between someone. Now let’s see between whom is this competition exactly going to be.

Competitive ELISA

Immunoglobulin A and IgA deficeincy mnemonic

IgA occurs as a monomer in the bloodstream and as a dimer when secreted (linked by the secretory component or a J chain attained from epithelial cells before secretion).

IgA is secreted onto mucosal surfaces (gastrointestinal, genitourinary, and respiratory) to block attachment of pathogens to mucous membranes.

Mnemonic: “ABCDE
A: Alone (Monomer)
B: in Blood
C: Component (Secretory component) or Chain (J chain) makes
D: Dimer
E: in Epithelial surfaces

What happens in IgA deficiency? Mnemonic!


Sandwich ELISA

To remember this type of ELISA, imagine a sandwich! The antigen is the filling and the antibodies are the yummy bread. Hungry, aren't you?

Sandwich ELISA

Indirect ELISA

Indirect ELISA is used for detection of a particular antibody in a given sample. Most of the HIV diagnosis kits use indirect ELISA principle.


Indirect ELISA

Direct ELISA

Direct ELISA is performed by attaching the sample antigens on a surface (walls of wells) then a specific antibody is applied so that it can bind to its corresponding antigen.

Direct ELISA

ELISA: Enzyme Linked Immuno Sorbent Assay

ELISA: Enzyme Linked Immuno Sorbent Assay

From pregnancy detection kits to HIV diagnosis it is ELISA which gives us appropriate results.  

Though ELISA has got a complex name, the way in which it diagnosis is really simple!

ELISA is performed in special micro titre plates which has wells in which we add our sample and then perform ELISA.

There are 4 types of ELISA:

- Sandwich
- Competitive

We’ll explain them one by one over the next few blogs. So stay tuned! :)

Written by Komal M. Kadam

Policemen of our body

Just like policemen save us from criminals, immune cells save our body!

T cell

Dendritic cells simplified

"Dendritic cells are messengers. I imagine them blowing loud sirens or ringing bells, alarming all the warriors to prepare for battle."

Dendritic cell

Interleukin 1 mnemonic


Interleukins
The name (IL) followed by a number (for example IL-l or IL-2) was coined in an attempt to develop a standardized nomenclature for molecules secreted by, and acting on, leukocytes.

IL-1: It is an acute phase reactant synthesized by macrophages contributing to the acute inflammatory response, including fever, leukocyte recruitment, adhesion molecule activation, and stimulation of further chemokine production.

Mnemonic: One makes you burn like the sun.

We are writing a book, Immunowesome and it will be available for download soon! Stay tuned :D

Neuron pencil: Sweet, sugar and blood

Diabetes needle pricks

Where do B cells and T cells reside in the lymph nodes and spleen?

Ever wondered where do these tiny little cells live in your body?
They live in organs called lymph nodes and spleen!

T cells and B cells in lymph node and spleen